ANTIHYPERTENSIVE EFFECTS OF HIBISCUS SABDARIFFA AND ZINGIBER OFFICINALE VAR. RUBRUM EXTRACTS IN A DEOXYCORTICOSTERONE ACETATE-SALT INDUCED HYPERTENSION RAT MODEL

This study evaluated the antihypertensive activity of the combination of Hibiscus sabdariffa Linn (roselle) extract and Zingiber officinale var.Rubrum (red ginger) extract on deoxycorticosterone-acetate-salt (DOCA-salt) induced hypertension in rats.Thirty male SD rats were randomly divided into six groups (n = 5 per group).Hypertension was induced by administering 30 mg/kg b.w.DOCA and 2% NaCl saline for three weeks.The treatment consisted of a normotensive group, a negative control (hypertension without treatment), a positive control (4.5 mg/200 g b.w.captopril orally) and three groups receiving the rosellered ginger extract combination (50:3.5 mg; 100:7 mg; and 200:14 mg)/300 g b.w. for two weeks.RAAS biomarkers and an inflammatory mediator were measured according to assay procedures.The blood pressure of rats treated with the roselle-red ginger combination extract decreased significantly (p < 0.05) compared to the negative control, with values approximating those of the normotensive group.Plasma renin level, serum ACE activity, plasma Ang-II level and IL-17A level also significantly declined in the combination extract group compared to the negative control group.The roselle-red ginger combination extract has antihypertensive activity due to its effects on blood pressure, RAAS biomarkers and inflammatory mediators in the hypertension model rats. RezumatAcest studiu a evaluat activitatea antihipertensiv a combinaiei de extracte de floare de roselle i ghimbir rou la obolani cu hipertensiune arterial indus de sarea DOCA.Treizeci de obolani masculi SD au fost mprii aleatoriu n ase grupuri (n = 5 per grup).Inducerea hipertensiunii arteriale a fost realizat prin administrarea a 30 mg/kg DOCA i soluie 2% NaCl timp de trei sptmni.Tratamentul grupului normotensiv, control negativ (hipertensiune arterial fr tratament), control pozitiv (4,5 mg/200 grame corp (gc) captopril pe cale oral) i 3 grupuri de combinaie de extract de roselle-ghimbir rou (50:3,5 mg; 100:7 mg; i 200:14 mg)/300 gc a fost efectuat timp de dou sptmni.Biomarkerii sistemului renin-angiotensin-aldosteron i mediatorii inflamatori au fost evaluai conform procedurilor de testare.Tensiunea arterial la obolanii tratai cu extract combinat de roselle-ghimbir rou a sczut semnificativ (p < 0,05) n comparaie cu grupul control negativ.n plus, nivelul reninei plasmatice, activitatea enzimei de conversie a angiotensinei serice, nivelul angiotensinei II n plasm i nivelul IL-17A au prezentat, de asemenea, o scdere semnificativ n grupul cu extract combinat n comparaie cu grupul martor negativ.