OXIDATIVE STRESS: MARKER OF ENDOTHELIAL DYSFUNCTION IN EXPERIMENTAL MODELS OF RATS WITH HYPERHOMOCYSTEINEMIA

The present experimental study aimed to evaluate the influence of hyperhomocysteinemia on endothelial dysfunction via the assessment of antioxidant activity in an experimental model of rats with hyperhomocysteinemia. Fourteen 6-month-old male Wistar rats weighing 450 -550 g were allocated into two groups.After 2 weeks of accommodation, both groups received High-Fat diet (HFD) for the entire study duration.The experimental group (G-II) was also administered methionine 1 mg/kg body weight orally daily during the first 20 days.Following model confirmation, G-II received atorvastatin 20 mg/kg body weight by daily gavage for the next 20 days.We evaluated, at the beginning and the end of the experimental study, the values for Homocysteine (Hcy) and parameters of oxidative stress (OS) -total antioxidant status (TAS), glutathione peroxidase (GPX) and superoxide dismutase (SOD).HFD induced significant lipid profile modification, while methionine induced hyperhomocysteinemia by altering the metabolism of Hcy.Atorvastatin treatment rescued both Hcy values and lipid profile in G-II.Our results show that atorvastatin treatment has lipid-lowering properties and antioxidative effects and also reduces the Hcy concentration in this experimental model of rats with hyperhomocysteinemia. Atorvastatin treatment proves promising in alleviating endothelial dysfunction and cardiovascular diseases. RezumatScopul acestui studiu a fost estimarea efectului hiperhomocisteinemiei asupra disfunciei endoteliale, prin intermediul evalurii statusului oxidativ, ntr-un model de roztor cu hiperhomocisteinemie indus artificial.14 obolani Wistar masculi, n vrst de 6 luni, cntrind ntre 450 i 550 g, au fost distribuii n dou grupuri.Dup 2 sptmni de acomodare, ambele grupuri au primit dieta bogat caloric (HFD) pe ntreaga perioad a desfurrii studiului.Grupul de studiu (G-II) a primit suplimentar metionin 1 mg/kg/zi, pe cale oral timp de 20 de zile.Dup confirmarea modelului, grupului G-II a fost administrat atorvastatin 20 mg/kg/zi, prin gavaj.Am evaluat, la nceputul i la sfritul studiului, valorile Hcy i parametrii stresului oxidativ (OS)statusul antioxidant total (TAS), glutation peroxidaza (GPX) i superoxid dismutaz (SOD).Dieta bogat caloric a generat modificri ngrijortoare asupra profilului lipidic, n timp ce metionina a indus hiperhomocisteinemie prin afectarea metabolismului Hcy.Tratamentul cu atorvastatin a determinat ameliorarea att a valorilor Hcy, ct i a profilului lipidic.Tratamentul cu atorvastatin are proprieti hipolipemiante, antioxidante i contribuie la scderea valorilor serice ale Hcy n acest model de obolani cu hiperhomocisteinemie. Prin urmare, atorvastatina se dovedete promitoare n scopul ameliorrii disfunciei endoteliale i a evenimentelor cardiovasculare.