Ultra-Specific G-Quadruplex–Colistin Interaction for Efficient Transcriptome-Wide G4 Mapping

G-quadruplexes (G4s) are challenging targets for chemical biology interventions, notably because of their dynamic topological polymorphism. We found that the antibiotic small- molecule colistin (COL) interacts specifically with a single subtype of G4 structures, the so-called parallel G4s. This interaction triggers the aggregation of the G4/COL complexes in a structure-specific manner, which can thus be separated from the bulk solution by centrifugation. This unprecedented mode of affinity-precipitation was exploited here to design the COL-induced RNA G4 precipitation and sequencing (CoRP-seq) protocol, which allows for the assessment of the prevalence of RNA G4s in the transcriptome of human cells in a straightforward manner. CoRP-seq shines by its ultraspecificity, simplicity, and practical convenience, which thus advances G4 mapping further and addresses unmet needs in the field of G4omics.

Authors

Institutions

Publication Details

Journal
Journal of the American Chemical Society
Published
2025-03-06
DOI
https://doi.org/10.1021/jacs.5c01172
Citations
6
Primary Topic
Advanced biosensing and bioanalysis techniques
Type
article
Field-Weighted Citation Impact
4.84

Funders

Controls
|||
ALL TIME
JAN
FEB
MAR
APR
MAY
JUN
JUL
AUG
SEP
OCT
article

Ultra-Specific G-Quadruplex–Colistin Interaction for Efficient Transcriptome-Wide G4 Mapping

Shijiong Wei, Xinrong Yan, Dehui Qiu, Huangxian Ju et al.
6 citations
Journal of the American Chemical Society
Advanced biosensing and bioanalysis techniques
4.84
article

Ultra-Specific G-Quadruplex–Colistin Interaction for Efficient Transcriptome-Wide G4 Mapping

Shijiong Wei, Xinrong Yan, Dehui Qiu, Huangxian Ju, Shentong Tao, Lionel Guittat, Jean‐Louis Mergny, David Monchaud, Jun Zhou, Wenli Zhang, Xiaobo Zhang, Guangming Li, Yilong Feng
article en
6 citations

Abstract

G-quadruplexes (G4s) are challenging targets for chemical biology interventions, notably because of their dynamic topological polymorphism. We found that the antibiotic small- molecule colistin (COL) interacts specifically with a single subtype of G4 structures, the so-called parallel G4s. This interaction triggers the aggregation of the G4/COL complexes in a structure-specific manner, which can thus be separated from the bulk solution by centrifugation. This unprecedented mode of affinity-precipitation was exploited here to design the COL-induced RNA G4 precipitation and sequencing (CoRP-seq) protocol, which allows for the assessment of the prevalence of RNA G4s in the transcriptome of human cells in a straightforward manner. CoRP-seq shines by its ultraspecificity, simplicity, and practical convenience, which thus advances G4 mapping further and addresses unmet needs in the field of G4omics.

Journal of the American Chemical SocietyVol. 147(11)
Nanjing Agricultural University (CN), École Polytechnique (FR), Université de Bourgogne (FR), Nord University (NO), Sorbonne Université (FR), Université Sorbonne Paris Nord (FR), Institut de Chimie (FR), Institut Polytechnique de Paris (FR), Institut de Chimie Moléculaire de l'Université de Bourgogne (FR), Université Bourgogne Europe (FR), Nanjing University (CN)
National Natural Science Foundation of China, State Key Laboratory of Analytical Chemistry for Life Sciences, Fundamental Research Funds for the Central Universities
Zero hunger
Openalex Percentile: Top 5%
Advanced biosensing and bioanalysis techniques
4.84
DOIOpen Access PDFOpenAlex
AI Navigator

Ask Laika to Summarize, Analyze, and Connect papers live on the map.

Summarize Papers & Methodologies

Extract key findings, datasets, and comparative methods across publications.

Benchmark Rankings & Visual Analytics

Rank top research institutions, authors, funders, topics, and journals by Field-Weighted Citation Impact (FWCI) and paper volume with instant charts.

Connect Distant Disciplines

Bridge topological clusters on the map to find hidden collaborative intersections.