Proteomic fingerprints of protein corona formation on MIL-88B(Fe)-NH2 metal-organic framework

• Protein corona of Fe-MOF in fish plasma was provided. • A thin shell of plasma corona was formed over the Fe-MOF surface. • The immunoglobulins and complement proteins were enriched on the surface of Fe-MOF. • The formation of protein corona on haemocompatibility of Fe-MOF was assessed When nanoparticles are introduced to living systems, they are quickly covered by a biomolecular coating referred to as the protein corona. This coating endows nanoparticles with new biorecognition properties, subsequently impacting their behavior and downstream toxic effects. Herein, the proteomic fingerprints of protein corona formation on Fe-based metal-organic framework (Fe-MOF) MIL-88B(Fe)-NH 2 exposed to plasma of the marine demersal teleost ( Sebastes schlegelii ) were investigated. The formation of fish plasma protein corona on haemocompatibility of Fe-MOF were investigated. Transmission electron microscopy and flow cytometry confirmed the formation of plasma protein coronas on the Fe-MOF particles. The immunoglobulins and complement proteins were enriched on the Fe-MOF surface, whereas the lipoproteins and coagulation proteins were significantly depleted compared with their original concentrations in the crude plasma. Hemolysis induced by Fe-MOF was influenced by fish plasma protein corona formation. This study offers insights into the interaction between a representative Fe-MOF and fish plasma proteins, enabling a better understanding of the biological behavior of Fe-MOFs in the blood system.