Relay-projection microscopic telescopy

The fundamental trade-off between spatial resolution and imaging distance poses a significant challenge for current imaging techniques, such as those used in modern biomedical diagnosis and remote sensing. Here, we introduce a new conceptual method for imaging dynamic amplitude-phase-mixed objects, termed relay-projection microscopic telescopy (rPMT), which fundamentally challenges conventional light collection techniques by employing non-line-of-sight light collection through square-law relay-projection mechanisms. We successfully resolved tiny features measuring 2.76 μm, 22.10 μm, and 35.08 μm for objects positioned at distances of 1019.0 mm, 26.4 m, and 96.0 m, respectively, from single-shot spatial power spectrum images captured on the relay screen; these results demonstrate that the resolution capabilities of rPMT significantly surpass the Abbe diffraction limit of the 25 mm-aperture camera lens at the respective distances, achieving resolution improvement factors of 7.9, 25.4, and 58.2. The rPMT exhibits long-distance, wide-range, high-resolution imaging capabilities that exceed the diffraction limit of the camera lens and the focusing range limit, even when the objects are obscured by a scattering medium. The rPMT enables telescopic imaging from centimeters to beyond hundreds of meters with micrometer-scale resolution using simple devices, including a laser diode, a portable camera, and a diffusely reflecting whiteboard. Unlike contemporary high-resolution imaging techniques, our method does not require labeling reagents, wavefront modulation, synthetic receive aperture, or ptychography scanning, which significantly reduce the complexity of the imaging system and enhance the application practicality. This method holds particular promise for in-vivo label-free dynamic biomedical microscopic imaging diagnosis and remote surveillance of small objects.

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Publication Details

Journal
Light Science & Applications
Published
2025-03-07
DOI
https://doi.org/10.1038/s41377-025-01800-6
Citations
1
Primary Topic
Advanced Fluorescence Microscopy Techniques
Type
article
Field-Weighted Citation Impact
1.63

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article

Relay-projection microscopic telescopy

Junli Qi, Mengjun Zhu, Shuyue Zhu, Luobing Dong et al.
1 citations
Light Science & Applications
Advanced Fluorescence Microscopy Techniques
1.63
article

Relay-projection microscopic telescopy

Junli Qi, Mengjun Zhu, Shuyue Zhu, Luobing Dong, Zhaohua Dong, Nan Gu, Xiujian Li, Meicheng Fu, Hongyu Zhang, Qiong Liu, Siyu Liu, Yi Zhang, Wenjun Yi, Yao Xu, Xin Chen, Ping Wang, Junyi Du, Peng Xiong
article en
1 citations

Abstract

The fundamental trade-off between spatial resolution and imaging distance poses a significant challenge for current imaging techniques, such as those used in modern biomedical diagnosis and remote sensing. Here, we introduce a new conceptual method for imaging dynamic amplitude-phase-mixed objects, termed relay-projection microscopic telescopy (rPMT), which fundamentally challenges conventional light collection techniques by employing non-line-of-sight light collection through square-law relay-projection mechanisms. We successfully resolved tiny features measuring 2.76 μm, 22.10 μm, and 35.08 μm for objects positioned at distances of 1019.0 mm, 26.4 m, and 96.0 m, respectively, from single-shot spatial power spectrum images captured on the relay screen; these results demonstrate that the resolution capabilities of rPMT significantly surpass the Abbe diffraction limit of the 25 mm-aperture camera lens at the respective distances, achieving resolution improvement factors of 7.9, 25.4, and 58.2. The rPMT exhibits long-distance, wide-range, high-resolution imaging capabilities that exceed the diffraction limit of the camera lens and the focusing range limit, even when the objects are obscured by a scattering medium. The rPMT enables telescopic imaging from centimeters to beyond hundreds of meters with micrometer-scale resolution using simple devices, including a laser diode, a portable camera, and a diffusely reflecting whiteboard. Unlike contemporary high-resolution imaging techniques, our method does not require labeling reagents, wavefront modulation, synthetic receive aperture, or ptychography scanning, which significantly reduce the complexity of the imaging system and enhance the application practicality. This method holds particular promise for in-vivo label-free dynamic biomedical microscopic imaging diagnosis and remote surveillance of small objects.

Light Science & ApplicationsVol. 14(1)
Xidian University (CN), National University of Defense Technology (CN), Chinese PLA General Hospital (CN), Hunan City University (CN), China XD Group (China) (CN)
National Natural Science Foundation of China, Natural Science Foundation of Hainan Province, National Key Research and Development Program of China
Openalex Percentile: Top 16%
Advanced Fluorescence Microscopy Techniques
1.63
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