Orthogonal set of engineered synapse organizers for multiplexed molecularly inducible neuron-microelectrode interfaces

Abstract One of the key limitations of microelectrode techniques for recording neuronal circuit activity is their lack of inherent selectivity for specific cell types. This issue is expected to be addressed by utilizing synapse organizers that induce synapse-like junctions onto the microelectrodes in a cell type-specific manner. In this study, we developed an orthogonal set of engineered synapse organizers with peptide ligands, each designed to be activated exclusively through interaction with its corresponding nanobody. These molecular tools would provide a foundation for a multiplexed, molecularly inducible neuron-microelectrode interface to access detailed neuronal circuit dynamics.