3D virtual histology of rodent and primate cochleae with multi-scale phase-contrast X-ray tomography

Multi-scale X-ray phase contrast tomography (XPCT) enables three-dimensional (3D), non-destructive imaging of intact small animal cochlea and apical cochlear turns. Here we report on post-mortem imaging of excised non-human primate and rodent cochleae at different [Formula: see text]-CT and nano-CT synchrotron instruments. We explore different sample embeddings, stainings and imaging regimes. Under optimized conditions of sample preparation, instrumentation, imaging protocol, and phase retrieval, high image quality and detail level can be achieved in 3D reconstructions. The showcased instrumentation and imaging protocols along with the reconstucted volumes can serve as benchmarks and reference for multi-scale microanatomy and 3D histology. The provided benchmarks and imaging protocols of this work cover a wide range of scales and are intended as augmented imaging tools for auditory research.

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Publication Details

Journal
Scientific Reports
Published
2025-03-07
DOI
https://doi.org/10.1038/s41598-025-89431-0
Citations
1
Primary Topic
Advanced X-ray Imaging Techniques
Type
article
Field-Weighted Citation Impact
1.88

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3D virtual histology of rodent and primate cochleae with multi-scale phase-contrast X-ray tomography

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Scientific Reports
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3D virtual histology of rodent and primate cochleae with multi-scale phase-contrast X-ray tomography

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article en
1 citations

Abstract

Multi-scale X-ray phase contrast tomography (XPCT) enables three-dimensional (3D), non-destructive imaging of intact small animal cochlea and apical cochlear turns. Here we report on post-mortem imaging of excised non-human primate and rodent cochleae at different [Formula: see text]-CT and nano-CT synchrotron instruments. We explore different sample embeddings, stainings and imaging regimes. Under optimized conditions of sample preparation, instrumentation, imaging protocol, and phase retrieval, high image quality and detail level can be achieved in 3D reconstructions. The showcased instrumentation and imaging protocols along with the reconstucted volumes can serve as benchmarks and reference for multi-scale microanatomy and 3D histology. The provided benchmarks and imaging protocols of this work cover a wide range of scales and are intended as augmented imaging tools for auditory research.

Scientific ReportsVol. 15(1)
German Primate Center (DE), European Synchrotron Radiation Facility (FR), Universitätsmedizin Göttingen (DE), Max Planck Institute for Multidisciplinary Sciences (DE), University of Göttingen (DE)
Georg-August-Universität Göttingen
Openalex Percentile: Top 20%
Advanced X-ray Imaging Techniques
1.88
DOIOpen Access PDFOpenAlex
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