Micropropagation of Plumbago auriculata plants by nodal segment explants

The study was conducted during the period from 2022 to 2024 at the Tissue Culture Laboratory in the Plant Genetic Resources Department at the Desert Research Center in Alexandria, where Plumbago auriculata Lam was micropropagated. The study aimed to develop a protocol for tissue culture propagation of Plumbago. Nodal segments were taken and cultured on MS medium in the presence of cytokinin (BA) at different concentrations: 0.0, 0.5, 1.0, 1.5, and 2.0 mg/L, in conjunction with auxin (IAA) at different concentrations: 0.0, 0.5, 1.0 and 1.5 mg/L for 30 days. A concentration of 1.5 mg/L of BA with a concentration of 1.00 mg/L of IAA resulted in the highest number of shoots and the longest shoot length. After the vegetative shoots reached a length of 5 cm, they were transferred to a rooting medium containing of plant growth regulators (IAA and IBA) concentration of 0.01 mg/l for 4 weeks, achieving a rooting percentage of 95%. Then the plants were transferred and planted in plastic containers containing a growth medium composed of peat moss and sand in a 1:1 ratio, and the acclimatization process was successfully completed with a 95%.