Rewiring Escherichia coli to transform formate into methyl groups
Within this study transformation of formate into methyl groups is demonstrated in E. coli. Our findings support that feeding formate can improve the availability of usable C<sub>1</sub>-compounds and, as a result, increase whole-cell methylation with engineered E. coli. Using this as a starting point, the introduction of additional auxiliary enzymes and ideas to make the system more energy-efficient are discussed for future applications.
Authors
- Steffen N. Lindner (ORCID: https://orcid.org/0000-0003-3226-3043)
- Jennifer N. Andexer (ORCID: https://orcid.org/0000-0001-8238-5749)
- Ari Satanowski (ORCID: https://orcid.org/0000-0003-0775-4799)
- Michael K. F. Mohr (ORCID: https://orcid.org/0009-0005-1322-178X)
- Tobias J. Erb (ORCID: https://orcid.org/0000-0003-3685-0894)
Institutions
- University of Freiburg (DE)
- Philipps University of Marburg (DE)
- Humboldt-Universität zu Berlin (DE)
- Max Planck Institute of Molecular Plant Physiology (DE)
- Loewe Center for Synthetic Microbiology (DE)
- Max Planck Institute for Terrestrial Microbiology (DE)
- Freie Universität Berlin (DE)
- Charité - Universitätsmedizin Berlin (DE)
Publication Details
- Journal
- Microbial Cell Factories
- Published
- 2025-03-07
- DOI
- https://doi.org/10.1186/s12934-025-02674-4
- Citations
- 6
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 4.84
Funders
- Deutsche Bundesstiftung Umwelt
- Deutsche Forschungsgemeinschaft
- European Research Council