Inconsistencies in the published rabbit ribosomal rRNAs: a proposal for uniformity in sequence and site numbering
Examination of all publicly available <i>Oryctolagus cuniculus</i> (rabbit) ribosome cryo-EM structures reveals numerous confusing inconsistencies. First, there are a plethora of single-nucleotide differences among the various rabbit 28S and 18S rRNA structures. Second, two nucleotides are absent from the NCBI Reference Sequence for the 18S rRNA gene. Moving forward, we propose using the Broad Institute's rabbit whole-genome shotgun sequence and numbering to reduce modeling ambiguity and improve consistency between ribosome models.
Authors
- Zuben P. Brown (ORCID: https://orcid.org/0000-0002-9846-2822)
- Raymond N. Burton‐Smith (ORCID: https://orcid.org/0000-0002-1535-6419)
- Christopher U.T. Hellen (ORCID: https://orcid.org/0000-0002-3982-2090)
- Yaser Hashem (ORCID: https://orcid.org/0000-0003-4264-5918)
- Swastik De (ORCID: https://orcid.org/0000-0002-9302-724X)
- Joachim Frank (ORCID: https://orcid.org/0000-0001-5449-6943)
- Tatyana V. Pestova (ORCID: https://orcid.org/0000-0003-3543-256X)
- Michelle Zhou
Institutions
- Université de Bordeaux (FR)
- Inserm (FR)
- SUNY Downstate Health Sciences University (US)
- National Institutes of Natural Sciences (JP)
- Columbia University Irving Medical Center (US)
- Southington Public School (US)
- Institut Européen de Chimie et Biologie (FR)
- Living Systems (United States) (US)
- Columbia University (US)
Publication Details
- Journal
- RNA
- Published
- 2025-03-06
- DOI
- https://doi.org/10.1261/rna.080294.124
- Citations
- 1
- Primary Topic
- RNA modifications and cancer
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Institutes of Health