Transcriptome analysis and reverse engineering verification of SNZ3Val125Ile and Pho3Asn134Asp revealed the mechanism of adaptive laboratory evolution to increase the yield of tyrosol in Saccharomyces cerevisiae strain S26-AE2
In this study, a strain, S26-AE2, with good growth and tyrosol production performance was obtained by adaptive evolution. The transcriptome analysis revealed that the differences in the expression of genes involved in metabolic pathways in adaptive evolutionary strains may be related to yeast growth and tyrosol production. Further reverse engineering verified that the mutation of SNZ3 promoted tyrosol synthesis in S. cerevisiae in glucose-rich medium. This study provides a theoretical basis for the metabolic engineering of S. cerevisiae to synthesise tyrosol and its derivatives.
Authors
- Linlong Xu (ORCID: https://orcid.org/0000-0003-4496-6571)
- Huili Xia
- Xiaoxue Yang (ORCID: https://orcid.org/0000-0003-0237-2492)
- Shihui Yang (ORCID: https://orcid.org/0000-0002-9394-9148)
- Jun Dai (ORCID: https://orcid.org/0000-0001-6143-4732)
- Kun Zhuang (ORCID: https://orcid.org/0000-0001-8540-4239)
- Siyao Liu (ORCID: https://orcid.org/0009-0009-7131-2187)
- Xiong Chen
- Lan Yao
- Na Song (ORCID: https://orcid.org/0000-0001-5140-1873)
Institutions
- Wuhan Polytechnic University (CN)
- Huanghuai University (CN)
- Hubei University of Technology (CN)
- Hubei University (CN)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2025-03-05
- DOI
- https://doi.org/10.1186/s13068-025-02627-4
- Primary Topic
- Microbial Metabolic Engineering and Bioproduction
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Hubei Provincial Department of Education
- National Natural Science Foundation of China
- Natural Science Foundation of Hubei Province