Protocol to modulate SUMOylation of a specific protein in budding yeast using chemical genetic approaches
SUMOylation (small ubiquitin-like modifier) is a ubiquitous and highly dynamic posttranslational modification. Here, we present a protocol to alter the local SUMOylation landscape of target proteins in budding yeast Saccharomyces cerevisiae using chemical genetic tools. We describe steps for recruiting SUMO enzymes (Ulp1<sup>PD</sup> or Ubc9) to GFP-tagged proteins using GBP (GFP-binding protein)-fusion proteins. We then detail procedures for inducing SUMO conjugation/deconjugation and the subsequent SUMOylation analysis. For complete details on the use and execution of this protocol, please refer to Gutierrez-Morton et al.<sup>1</sup>.
Authors
- Yanchang Wang (ORCID: https://orcid.org/0000-0001-6782-0570)
- Emily Gutierrez-Morton
Institutions
- Florida State University (US)
- Florida A&M University - Florida State University College of Engineering (US)
Publication Details
- Journal
- STAR Protocols
- Published
- 2025-03-01
- DOI
- https://doi.org/10.1016/j.xpro.2025.103675
- Citations
- 1
- Primary Topic
- Ubiquitin and proteasome pathways
- Type
- article
- Field-Weighted Citation Impact
- 0.81
Funders
- National Institutes of Health
- National Institute of General Medical Sciences