Synthesis and Characterization of Polycaprolactone Scaffolds Containing Carbon Quantum Dots Nanoparticles for Tissue Engineering Applications

Background: Tissue engineering, by designing biological scaffolds and imitating the extracellular environment, helps the growth and proliferation of cells and plays a key role in replacing and repairing damaged tissues.In recent years, the addition of nanoparticles, such as carbon quantum dots, to biological scaffolds has received attention.In this research, the synthesis of polycaprolactone scaffolds containing carbon quantum dots and the investigation of biocompatibility effects and their protection have been discussed.Methods: Carbon quantum dots were synthesized using the pyrolysis method, and polymer scaffolds containing carbon quantum dots were prepared by the electrospinning method.The physical and chemical properties of the scaffold were evaluated by scanning electron microscopy and FTIR spectroscopy.The scaffolds' biocompatibility and antioxidant properties were measured by the MTT method.Results: Examination of the morphology and chemical showed the appropriate porosity of the scaffold containing carbon quantum dots.The MTT assay significantly enhanced stem cell viability on scaffolds incorporating carbon quantum dots.Furthermore, these scaffolds exhibited a significant protective effect against oxidative stress.Conclusion: This study showed that the polycaprolactone scaffold containing carbon quantum dots, with high biocompatibility and suitable antioxidant properties, provides an effective substrate for tissue engineering and cell protection under oxidative stress conditions.