Study the Isolation of mesenchymal stem cells from newly born infants of the fetal-chorionic plate

Abstract Stem cells are exclusive population of cells located in all stages of lifetime that holds capability to self-renewal plus specialize to group of cell lineages of multiple organs and tissues and considered a major source of differentiating cell types in the body, stem cells has been considered as part of new cell therapy of numerous damaged organs for instant embryonic stem cells in term of neurological cases like stroke and parkinson’s as these cells are transplanted in the body for focal organ damage repair, differentiation and immunoregulatory response, chemoattractant and stimulate tissue regeneration Current study detected the presence of mesenchymal stem cells isolated from Iraqi newborn infants placenta using enzymatic method of collagenase I enzyme and cultured with DMEMlow glucose, through observation of their genetic markers expression such as CD105, CD90 and CD73 using RT-PCR showed positively folding as well as the onset of their expression levels reduction as an initiation process for osteoblastic gene expression using epidermal growth factor plus osteogenic differentiation factors including β-glycerophosphate disodium salt pentahydrate, Dexamethasone and L-ascorbic acid