Establishment and application of an RNAi system in Pichia pastoris
This study demonstrates the successful establishment of an RNAi system in <i>P. pastoris</i>, enabling efficient gene suppression for metabolic engineering. RNAi offers a faster and more efficient method for regulating gene expression, improving heterologous protein secretion and 3-HP production. This system is a valuable tool for optimizing <i>P. pastoris</i> as a microbial cell factory, with strong potential for industrial applications.
Authors
- Aoxue Wang (ORCID: https://orcid.org/0000-0003-2223-3561)
- Chenfeng He
- Shuli Liang (ORCID: https://orcid.org/0000-0002-9224-0049)
- Shupeng Ruan
- Ying Lin
Institutions
- South China University of Technology (CN)
Publication Details
- Journal
- Frontiers in Bioengineering and Biotechnology
- Published
- 2025-03-05
- DOI
- https://doi.org/10.3389/fbioe.2025.1548187
- Citations
- 2
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 1.61