Biodistribution and persistence of human umbilical cord-derived mesenchymal stem cells in NCG mice: a comparative study
Each method exhibited strengths and weaknesses: <sup>89</sup>Zr-PET/CT enabled long-term tracking but encountered issues with <sup>89</sup>Zr shedding and dead cells; bioluminescence provided specific detection but was hampered by a rapid decline in signal; mIHC identified cells but relied on antigen abundance; qPCR detected minimal cell quantities but was unable to differentiate between live and dead cells. These limitations may obscure the true fate of cells <i>in vivo</i>, highlighting the need for more accurate and reliable assessment techniques.
Authors
- Yongjun Liu (ORCID: https://orcid.org/0000-0002-0166-6942)
- Haomiao Long
- Herui Wang
- Chenliang Zhang (ORCID: https://orcid.org/0000-0003-2274-2657)
- Guangyang Liu (ORCID: https://orcid.org/0000-0003-4569-2197)
- Miao Li (ORCID: https://orcid.org/0000-0001-6672-8692)
- Xin Li (ORCID: https://orcid.org/0000-0001-8328-4894)
- Qinggang Ge (ORCID: https://orcid.org/0000-0002-0305-689X)
- Yaoyao Chen (ORCID: https://orcid.org/0009-0006-8897-5284)
- Jun He (ORCID: https://orcid.org/0000-0002-2337-0405)
- Yi Mi
Institutions
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Peking University (CN)
- Institute of Laboratory Animal Science (CN)
- Peking University Third Hospital (CN)
Publication Details
- Journal
- Future Science OA
- Published
- 2025-03-04
- DOI
- https://doi.org/10.1080/20565623.2025.2471723
- Citations
- 1
- Primary Topic
- Mesenchymal stem cell research
- Type
- article
- Field-Weighted Citation Impact
- 1.63
Funders
- National Key Research and Development Program of China