Changes in the motifs in the D0 and SD2 domains of the S protein drive the evolution of virulence in enteric coronavirus porcine epidemic diarrhea virus

The continuous emergence of novel viral variants in the current landscape poses challenges for disease prevention and control. Before 2010, PED caused by GI strains was only sporadic outbreaks and not large-scale epidemics. Since 2010, highly virulent GII strains derived from GI strains have spread worldwide and caused significant economic losses. However, the molecular mechanism underlying the differences in virulence is still unclear. In this study, the differences in the predicted glycosylation sites of the S protein between the GI and GII strains were taken as the starting point to explore the key sites responsible for the variations in PEDV virulence. The results indicate that the motifs 57ENQGVNST64 and 722NSTF725 of the S protein in the GII strains are involved in the evolution of PEDV virulence. This study provides a new perspective on the molecular mechanism of PEDV virulence evolution.