Comparison of broth microdilution and Etest® methods for susceptibility testing of amphotericin B in <i>Candida auris</i>
Amphotericin B remains an important treatment for multidrug-resistant Candida auris. Antifungal susceptibility testing of amphotericin B in C. auris can vary depending on the methodology used. Here, we compared the Etest method and the Clinical and Laboratory Standards Institute broth microdilution reference method for amphotericin B against 60 clinical C. auris isolates from the four major clades. The minimum inhibitory concentrations differed significantly by method (P-value, <.0001), and discrepancies were observed in the interpretation of resistance (categorical agreement, 88.3%; very major error, 33.3%). Broth microdilution may represent a more conservative approach for detecting amphotericin B resistance in C. auris.
Authors
- Tanis C. Dingle (ORCID: https://orcid.org/0000-0002-6936-406X)
- Philippe J. Dufresne (ORCID: https://orcid.org/0000-0001-5871-3933)
- Linda Hoang (ORCID: https://orcid.org/0000-0001-7769-6120)
- Amrita Bharat (ORCID: https://orcid.org/0000-0003-4171-9436)
- Julianne V. Kus (ORCID: https://orcid.org/0000-0001-6033-7244)
- Xiao Rui Li
- Caroline Sheitoyan-Pesant
- David C. Alexander (ORCID: https://orcid.org/0000-0003-3509-445X)
- Domenica G. De Luca (ORCID: https://orcid.org/0009-0008-3829-8873)
Institutions
- Manitoba Health (CA)
- University of Calgary (CA)
- Public Health Agency of Canada (CA)
- Toronto Public Health (CA)
- University of Toronto (CA)
- BC Centre for Disease Control (CA)
- Provincial Laboratory of Public Health (CA)
- Public Health Ontario (CA)
- Ste. Anne's Hospital (CA)
- Dr. Georges-L.-Dumont University Hospital Centre (CA)
- University of Manitoba (CA)
Publication Details
- Journal
- Medical Mycology
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1093/mmy/myaf019
- Citations
- 1
- Primary Topic
- Antifungal resistance and susceptibility
- Type
- article
- Field-Weighted Citation Impact
- 1.40