Genome-wide identification of the UGT genes family in Acer rubrum and role of ArUGT52 in anthocyanin biosynthesis under cold stress

Acer rubrum is a widespread Acer species valued for its vibrant autumn foliage. The UGT (UDP-glycosyltransferase) gene family is integral to the biosynthesis of anthocyanins, the pigments responsible for leaf coloration. This study aimed to comprehensively identify and characterize the UGT gene family in the A. rubrum genome. The results of the phylogenetic analysis of 249 ArUGTs revealed 18 distinct subgroups. Conserved motif analysis demonstrated structural similarities within subgroups. Gene duplication analysis identified 21 tandem and 66 segmental duplication events across chromosomes. Transcriptomic data from autumn leaves of different colours and under low-temperature stress were analyzed for ArUGT expression patterns. Compared to controls, 44 UGTs were upregulated and 99 downregulated in yellow leaves, while 59 were upregulated and 84 downregulated in red leaves. Low-temperature treatments showed upregulation of 18 UGTs at 10 °C and 40 UGTs at 4 °C. Downregulation was observed in 7 UGTs at 10 °C and 33 UGTs at 4 °C. Among all UGT genes, ArUGT52 was common in highly expressed genes in both red leaf and low-temperature stress. Furthermore, the transient overexpression of ArUGT52 in tobacco plants demonstrated cytoplasmic localization and a marked increase in anthocyanin levels under cold stress. In vitro, biochemical assay results indicated that the ArUGT52 was involved in anthocyanin biosynthesis via the glucosylation of anthocyanidins. This study provides insights into the genetic mechanisms of leaf coloration and the potential of UGT manipulation for enhancing plant responses to low-temperature stress. These findings have applications in ornamental horticulture and agriculture.