Cryo-STEM tomography for cell biology using thick lamella
Electron tomography is a powerful tool for structural studies in cell biology, but specimen thickness remains a significant limitation. Scanning transmission electron microscopy (STEM) tomography offers advantages in this regard. Recent developments in focused ion beam slicing methods for cryo-cell biology have enabled the observation and 3D reconstruction of relatively thick specimens (300-500 nm) using cryo-STEM tomography. Organelles such as mitochondria and the nuclear membrane have been clearly reconstructed, demonstrating the promise of STEM tomography for structural studies in cell biology.
Authors
- Hiroko Takazaki
- Sayaka Kawai (ORCID: https://orcid.org/0009-0009-2984-5094)
- Kazuhiro Aoyama
- Misaki Arie
- Hironori Suemune
Institutions
- Thermo Fisher Scientific (Japan) (JP)
- Sony Computer Science Laboratories (JP)
- Protein Research Foundation (JP)
- The University of Osaka (JP)
Publication Details
- Journal
- Microscopy
- Published
- 2025-03-04
- DOI
- https://doi.org/10.1093/jmicro/dfaf017
- Primary Topic
- Advanced Electron Microscopy Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00