Single-cell transcriptomics of clinical grade adipose-derived regenerative cells reveals consistency between donors independent of gender and BMI

Adipose-derived regenerative cells (ADRCs) also referred to as the stromal vascular fraction, provide an ample source of stem cells with widespread regenerative therapeutic use. Being heterogenous in nature, possibly affecting the clinical outcome after stem cell treatment, the ADRC- donor, -BMI, and -gender may have a large impact on ADRC composition and quality but this remains largely unexplored. Herein, we provide a comprehensive single-cell RNA sequencing ADRC mapping across two cell trial intervention studies but found no gender- or BMI-related variations, except for a minor female increase in PI16/CD55-expressing stem cells. Indeed, ADRC heterogeneity was surprisingly minimal between donors. This provides important decision-making support on adipose stem cell donor selection for stem cell treatments, and suggest that donor, gender and BMI should be regarded as less influential.

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Publication Details

Journal
Stem Cell Research & Therapy
Published
2025-03-05
DOI
https://doi.org/10.1186/s13287-025-04234-4
Citations
4
Primary Topic
Single-cell and spatial transcriptomics
Type
article
Field-Weighted Citation Impact
3.22

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article

Single-cell transcriptomics of clinical grade adipose-derived regenerative cells reveals consistency between donors independent of gender and BMI

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4 citations
Stem Cell Research & Therapy
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article

Single-cell transcriptomics of clinical grade adipose-derived regenerative cells reveals consistency between donors independent of gender and BMI

Jakob V. Nielsen, Charlotte Harken Jensen, Lars Lund, Pratibha Dhumale, Mads Gustaf Jørgensen, Mark Burton, Jens Ahm Sørensen, Frederik Adam Bjerre, Ditte Caroline Andersen, Sabrina Toft Hansen, Mads Thomassen
article en
4 citations

Abstract

Adipose-derived regenerative cells (ADRCs) also referred to as the stromal vascular fraction, provide an ample source of stem cells with widespread regenerative therapeutic use. Being heterogenous in nature, possibly affecting the clinical outcome after stem cell treatment, the ADRC- donor, -BMI, and -gender may have a large impact on ADRC composition and quality but this remains largely unexplored. Herein, we provide a comprehensive single-cell RNA sequencing ADRC mapping across two cell trial intervention studies but found no gender- or BMI-related variations, except for a minor female increase in PI16/CD55-expressing stem cells. Indeed, ADRC heterogeneity was surprisingly minimal between donors. This provides important decision-making support on adipose stem cell donor selection for stem cell treatments, and suggest that donor, gender and BMI should be regarded as less influential.

Stem Cell Research & TherapyVol. 16(1)
University of Southern Denmark (DK), Odense University Hospital (DK)
Innovationsfonden, Novo Nordisk, Odense Universitetshospital, Syddansk Universitet, Novo Nordisk Fonden, Klinisk Institut, Syddansk Universitet
Gender equality
Openalex Percentile: Top 9%
Single-cell and spatial transcriptomics
3.22
DOIOpen Access PDFOpenAlex
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