GREEN SYNTHESIZED SILVER NANOPARTICLES USING LAI (DURIO KUTEJENSIS) LEAVES INFUSION WATER AND ITS INHIBITION ACTIVITY AGAINST ESCHERICHIA COLI FOR WASTEWATER TREATMENT APPLICATION

Silver nanoparticles (AgNP) have piqued the interest of researchers due to their antibacterial and antimicrobial properties, which are helpful in wastewater treatment applications. Green synthesis was selected because it is simple, quick, inexpensive, low in energy, and environmentally friendly. Many leaves have been studied in research, but the Lai leaves (Durio kutejensis), a plant endemic to Kalimantan, has yet to be studied. The leaves contain phenolic compounds, which act as reducing agents and stabilizers during the synthesis of AgNP. The success of AgNP formation is dependent on several process parameters, including pH and temperature. In brief, a 2:1 (v/v) ratio of AgNO3 [0.001M] and Lai leaves infusion water was used in the synthesis. The pH levels were set to 5, 8, and 11, and the temperatures were set to room temperature and 80 °C. UV-Vis and a particle size analyzer (PSA) will be used to characterize the synthesized result. In an alkaline environment, the UV-Vis spectrum shows the peak in all samples. In addition, the highest temperature produced the highest peak at 413-415 nm wavelength, indicating the formation of AgNP. The PSA revealed that all samples produced a homogeneous size because they had a polydispersity index of less than 0.5 with nanoparticle sizes ranging from 1 to 18 nm. In addition, the FTIR analysis of Lai leaves revealed the presence of hydroxyl and carbonyl groups, which play a role in the reduction and stabilization of nanoparticles. Subsequently, the antibacterial properties of AgNP were also investigated using the well-diffusion antibacterial test, which was observed every 24 hours for three days. The optimum zone of inhibition was yielded at alkaline and room temperature due to its nano-sized particles. Therefore, green synthesis using Lai leaves is the potential to produce AgNP with suitable antibacterial activities