Fluorescent antibody-based detection and ultrastructural analysis of Streptococcus pneumoniae in human sputum
Spn-FLUO is a rapid and sensitive assay for detecting Spn in human sputum within 30 min, encompassing a range of both vaccine and non-vaccine serotypes associated with pneumococcal pneumonia. The study highlights that most pneumococci form aggregates in human sputum.
Authors
- Michael J. Francis (ORCID: https://orcid.org/0000-0002-5433-0211)
- Maneesha Chitanvis (ORCID: https://orcid.org/0000-0001-8159-0038)
- Claudio F. Lanata
- William P. Daley
- Pierre Vidal
- Ana G. Jop Vidal (ORCID: https://orcid.org/0009-0005-0785-0889)
- I. J. Frame (ORCID: https://orcid.org/0000-0002-7560-7427)
- Carlos Gabriel Grijalva (ORCID: https://orcid.org/0000-0002-2329-7797)
- Kenichi Takeshita (ORCID: https://orcid.org/0000-0002-0869-2342)
- Poonam Sharma (ORCID: https://orcid.org/0000-0003-0668-3124)
- Jorge Eugenio Vidal (ORCID: https://orcid.org/0000-0003-0573-5658)
Institutions
- Emory University (US)
- Jackson Memorial Hospital (US)
- University of Mississippi Medical Center (US)
- Instituto de Investigación Nutricional (PE)
- Quest Diagnostics (United States) (US)
- Vanderbilt University Medical Center (US)
Publication Details
- Journal
- Pneumonia
- Published
- 2025-03-05
- DOI
- https://doi.org/10.1186/s41479-025-00157-z
- Citations
- 5
- Primary Topic
- Pneumonia and Respiratory Infections
- Type
- article
- Field-Weighted Citation Impact
- 5.42
Funders
- National Institutes of Health