A Cutting-edge Innovation: Europium-doped Monetite-infused Carrageenan – Hyaluronic Acid Membrane Enhances Soft-tissue Regeneration

Introduction: Biopolymers, known for their biocompatibility and ability to mimic the extracellular matrix (ECM), hold great potential in soft-tissue regeneration. Challenges in traditional treatments for gingival recession have driven the exploration of alternative scaffolding materials. A promising approach involves the fabrication of a Europium-Monetite-infused Carrageenan-Hyaluronic Acid (CAR +HA+ Eu-Monetite) membrane to enhance mechanical strength, improve stability, and promote tissue regeneration. Materials and Methods: The scaffold was synthesised by blending europium-monetite into a CAR + HA matrix. Characterisation techniques included scanning electron microscopy (SEM) for surface morphology, Fourier-transform infrared (FTIR) spectroscopy for chemical composition, and tensile testing for mechanical properties. Contact angle measurements evaluated surface wettability, while haematoxylin and eosin and Masson’s trichrome staining were conducted at 7 and 14 days to assess tissue organisation and ECM formation. Results: SEM revealed a porous structure in the europium-monetite scaffold, enhancing cellular interactions. FTIR confirmed the presence of key functional groups, while tensile testing showed significant improvements in strength and load-bearing capacity. Contact angle measurements indicated reduced hydrophilicity with the addition of europium-monetite. Histological analysis revealed denser tissue organisation, enhanced ECM formation, and improved collagen deposition in the test group compared to the control. Discussion: The europium-doped monetite scaffold demonstrated enhanced structural, mechanical, and biological properties. It promoted collagen synthesis and ECM remodelling, fostering better tissue integration. The study highlights europium’s role in angiogenesis and tissue regeneration, suggesting the scaffold’s potential for clinical applications in soft-tissue repair. Conclusion: The CAR + HA + Eu-monetite scaffold holds promise as a viable alternative for soft-tissue regeneration, offering improved mechanical and biological performance.