A Study of Gene Expression of Inhibin and Activin Subunits (INHA,INHBA) in the Placenta of Women with Missed Miscarriage

Missed Miscarriage (MM) was a special type of spontaneous Miscarriage that the embryo orfetus has already died but remained in the uterus for days or weeks and with a closed cervicalostium . Patients might present with or without subtle clinical symptoms such as vaginal bleedingor abdominal pain. Missed Miscarriage, occuring in approximately 8–20% of clinically confirmedintrauterine pregnancies, was often confirmed using ultrasonography. In this study, We used bioinformatics analysis to identify hub genes. Verified the expressionof the genes inhibin subunit alpha(INHA) and inhibin subunit beta A(INHBA ) in women placentawith MM .Placental tissues were obtained from therapeutic abortion (8-16 weeks, first trimester)and it was kepped in shield solution at -20 c. Genomic RNA from tissues samples were extractedby using Direct-zol™ RNA MiniPrep Kit(USA),then INHA and INHBA genes was amplifiedthrough via Reverse Transcription-Pol merase Chain Reaction(RT-PCR).We used The Livakmethod, also known as the 2−ΔΔCt method, is a widely used approach for analyzing data fromquantitative real-time PCR (qPCR) experiments to calculate changes in gene expression levels