GC-MS Analysis, Antipyretic, and COX- 2 Inhibitory Activity of Traditional Fermented Formulation Ushirasav

Ushirasav is a fermented traditional polyherbal formulation of modern Ayurveda, it is used to treat heat generate disorders in the body like bleeding of the nose and rectum, in this research Ushirasav is prepared according to the method given in Ayurvedic pharmacopeia of India by a controlled fermentation process and it was strictly observed for the presence of ethanol in a prescribed amount and the absence of methanol. Then Ushirasav was exposed to GC-MS analysis for exploration of types of metabolites present in the formulation and their reported effects, based on traditional claims, Ushirasav was subjected to in vivo antipyretic potential. It was found that Ushirasav showed antipyretic potential as compared to standard drug Paracetamol. Then by GCMS, analysis the prime content of Ushirasav is found as Benzoic acid. Benzoic acid was docked on Cyclooxygenase enzyme II for exploration of antipyretic potential.Benzoic acid bind with COX II and its binding energy were comparable to Indomethacin. Thus, it was explored from this research that Ushirasav shows remarkable antipyretic activity and it may be attributed to presence of Benzoic acid.