Novel RP-UFLC Methodology for Concurrent Assessment of Curcumin, Resveratrol, Silybin, and Mangiferin From Topical Hydrogels

Background: In topical preparations, four herbal compounds, namely mangiferin (MF), resveratrol (RV), silybin (SB), and curcumin (CR) need to be quantified quickly and accurately.Objective: This study utilizes a Franz diffusion cell setup to evaluate the suitability of reversed-phase ultra-fast liquid chromatography (UFLC) for assessing MF, RV, SB, and CR in a mouse skin.Methods: A C-18 column (2504.6 mm, 5 m) was used at 40 C for chromatographic separation.The mobile phase, a gradient of acetonitrile and 0.1% formic acid in highperformance liquid chromatography (HPLC) grade water was pumped at a rate of 1 mL per min.A 20 L sample was loaded, and analytes were accessed using a photodiode array (PDA) detector.The method validation included selectivity, linearity (2-20 g/mL), accuracy, precision, robustness, and sensitivity.The method's run time was 20 min. Results:The UFLC method was selective for the given compounds, and over the concentration range of 2-20 g/mL, the method's linearity was demonstrated with a correlation coefficient >0.99.Additionally, it was accurate, precise, robust, and sensitive, with a total run time of 20 min. Conclusion:The validated reversed-phase UFLC method meets the highlighted need for accurate and quick measurement of MF, RV, SB, and CR in topical formulations.Its effective validation and application in researching drug features using a Franz diffusion cell apparatus make it an important analytical tool in pharmaceutical research, ensuring precise drug content, homogeneity, and release properties in topical formulations.