A nanovaccine for immune activation and prophylactic protection of atherosclerosis in mouse models
Vaccines offer prophylactic treatments against atherosclerosis by eliciting effector T cell and antibody responses, which require effective delivery of antigen and adjuvant to activate dendritic cells (DC). Here we show that individual conjugation of antigen p210 and adjuvant CpG oligodeoxynucleotides onto superparamagnetic iron oxide nanoparticles formulates a nanovaccine cocktail that activates DCs for antigen cross-presentation and induction of co-stimulatory signals, cytokines and CD8<sup>+</sup> effector/effector memory T cell responses. This nanovaccine modulates the DCs in the draining lymph nodes, activates both CD4<sup>+</sup> and CD8<sup>+</sup> T cells, elicits memory responses, and induces both anti-p210 IgM and IgG antibodies to suppress atherosclerosis. Lastly, three intradermal vaccinations of this nanovaccine mitigate the atherosclerosis development in the ApoE<sup>-/-</sup> mice. Our nanovaccine design and preclinical data thus presents a potential candidate for prophylactic treatment for atherosclerosis.
Authors
- Xiaoling Ma (ORCID: https://orcid.org/0000-0002-2732-8669)
- Lei Zhang (ORCID: https://orcid.org/0000-0002-9312-8278)
- Weixiao Ding (ORCID: https://orcid.org/0000-0003-0087-0324)
- Xinmeng Li (ORCID: https://orcid.org/0000-0002-6863-6078)
- Wanying Zhu (ORCID: https://orcid.org/0000-0002-6292-4108)
- Dan-Xuan Zhu
- Dongping Sun (ORCID: https://orcid.org/0000-0001-5555-1588)
- Chuntao Chen (ORCID: https://orcid.org/0000-0003-4763-3211)
- Chao Zuo (ORCID: https://orcid.org/0000-0002-1461-0032)
- Abdulrahman AL-Ammari
- Hongsong Zhang (ORCID: https://orcid.org/0000-0003-4251-7609)
- Peng Zhou (ORCID: https://orcid.org/0000-0001-9863-4201)
- Jiaan Zhang (ORCID: https://orcid.org/0000-0002-3984-3860)
- Xu Zhi
- Qingqing Yu
- Y. Gai
Institutions
- University of Science and Technology of China (CN)
- Chinese Academy of Medical Sciences & Peking Union Medical College (CN)
- Nanjing University of Science and Technology (CN)
- Jiangsu Province Hospital (CN)
- Nanjing Medical University (CN)
- Nanjing University (CN)
Publication Details
- Journal
- Nature Communications
- Published
- 2025-03-02
- DOI
- https://doi.org/10.1038/s41467-025-57467-5
- Citations
- 9
- Primary Topic
- Atherosclerosis and Cardiovascular Diseases
- Type
- article
- Field-Weighted Citation Impact
- 7.18
Funders
- National Natural Science Foundation of China
- Natural Science Foundation of Jiangsu Province