Habitat-Dependent DNA viral communities in atmospheric aerosols: Insights from terrestrial and marine ecosystems in East Asia

The transmission of viruses through aerosols is of growing public health concern, yet research on aerosol-associated viral communities lags behind that of terrestrial and aquatic ecosystems. Here, DNA viral diversity in natural aerosols from both over land and ocean in the East Asia region was examined. The results showed that atmospheric environments harbor a distinct viral community that differs from those present in terrestrial and aquatic ecosystems. A comparison of aerosol samples from different locations revealed that aerosol viruses are strongly influenced by altitude and their sources. Fragments of viruses that can infect pathogenic bacteria, as well as pathogenic viruses (such as herpesviruses, Inoviruses, and Iridovirus) were detected. Anthropogenically-influenced land aerosol samples contained viral communities with greater richness and diversity as well as a higher relative abundance of pathogenic and lytic viruses compared to pristine marine airborne samples. Furthermore, habitat-specific auxiliary metabolic genes (AMGs) were observed, such as the phosphate regulon (phoH), which was more prevalent in ocean aerosol samples and regulates phosphate uptake under low-phosphate conditions, thereby assisting viral hosts in overcoming metabolic challenges in different environmental conditions. This study highlights the ecological distinctness of the airborne viral community and the interconnectedness between those from land, sea, and atmosphere, underscoring the importance of evaluating their potential pathogenicity in future research.