Biochemical and histological analysis of collagen content in lungs, liver and kidneys of rats treated with Beta vulgaris L. var. cicla

Objective: Collagen is a fundamental component of the extracellular matrix (ECM) and plays a critical role in organ structure, cellular functions, and wound healing. Beta vulgaris L. var. cicla (chard) is known for its diverse bioactive compounds, including vitamins, flavonoids, and nitrates. Chard has been associated with numerous health benefits, such as antioxidant, anti-inflammatory, and antidiabetic effects. This study investigates the impact of chard on collagen content in vital organs, specifically the lungs, liver, and kidneys. Methods: The rats divided into two groups: the control and the chard given group. The chard extract was administered to rats at a dose of 100 mg/kg per day for 7 days. On the 8th day, the rats were sacrificed, and tissues from the lungs, kidneys, and liver were collected. The collagen content was measured using both biochemical and histological analyses. Results: Chard administration exhibited tissue-specific effects on collagen content: it increased collagen in the lungs, decreased it in the liver significantly, and had no effect on kidney collagen. These biochemical changes were supported by histological results in the lungs and kidneys; however, no significant histological changes were observed in the liver. These varied effects might be related to differences in collagen metabolism and regulatory mechanisms across tissues. Conclusion: The findings suggest that chard, due to its distinct effects on collagen synthesis and ECM remodeling, holds promise as a potential therapeutic agent for applications such as wound healing, tissue strengthening, and antifibrotic therapy. Further studies on the mechanisms underlying these effects are necessary to fully understand the potential of chard in clinical applications.