Simultaneous Quantification of Multi-Class Antimicrobials in Chicken Kidney and Liver by New Validated UPLC-MS/MS Method

A novel and significant method was developed and validated with a sensitive, rapid, and simultaneous analytical method to determine antimicrobials in chicken tissues such as the kidney and liver. The process involved a unique approach to precipitation extraction. This method has not been widely used in this context, followed by the evaporation of the supernatant and reconstitution with the mobile phase. Antimicrobials, including Azithromycin, Clarithromycin, Erythromycin, Clavulanic acid, Ciprofloxacin, Clofazimine, Fluconazole, Linezolid, and Moxifloxacin were meticulously considered for development and validation in the chicken tissues. These antimicrobials were chosen based on their everyday use in poultry farming and their potential impact on human health. We used Ultra-Performance Liquid Chromatography with triple quad Mass Spectrometry and employed multiple reaction monitoring to detect the analytes of interest. All the compounds were well separated using Atlantis T3, 4.6x50mm, 3 μm. The linear range was set between 25 to 1000 ng/gm. The method was validated following linearity, extraction recovery, matrix effect impact, limit of detection, sensitivity, autosampler and benchtop stability, ensuring the results’ reliability and our method’s robustness.