Nanoscale Biodegradable Printing for Designed Tuneability of Vaccine Delivery Kinetics
Two-photon polymerization (2PP) 3D printing enables top-down biomaterial synthesis with nanoscale spatial resolution for de novo design of monodisperse injectable drug delivery systems. Spatiotemporal Controlled Release Inks of Biocompatible polyEsters (SCRIBE) is a novel poly(lactic-co-glycolic acid)-triacrylate resin family with sub-micron resolution and tuneable hydrolysis that addresses the limitations of current 2PP resins. SCRIBE enables the direct printing of hollow microparticles with tuneable chemistry and complex geometries inaccessible to molding techniques, which are used to engineer controlled protein release in vitro and in vivo. SCRIBE microparticles are used to modulate antibody titers and class switching as a function of antigen release rate and extend these findings to enable a single-injection vaccine formulation with extended antibody induction kinetics. Demonstrating how the chemistry and computer-aided design of 2PP-printed microparticles can be used to tune responses to biomacromolecule release in vivo opens significant opportunities for a new generation of drug delivery vehicles.
Authors
- Giulia Brachi (ORCID: https://orcid.org/0000-0002-0785-2332)
- Kun Zhou (ORCID: https://orcid.org/0000-0003-4109-5926)
- Patrick Peschke (ORCID: https://orcid.org/0009-0006-4097-7256)
- Omar Rifaie‐Graham (ORCID: https://orcid.org/0000-0003-1403-0537)
- David J. Peeler (ORCID: https://orcid.org/0000-0003-2441-6409)
- Xin Song (ORCID: https://orcid.org/0000-0003-4017-9685)
- Rujie Sun (ORCID: https://orcid.org/0000-0001-6136-7277)
- John S. Tregoning (ORCID: https://orcid.org/0000-0001-8093-8741)
- Jonathan Yeow (ORCID: https://orcid.org/0000-0003-3709-5149)
- Paul F. McKay (ORCID: https://orcid.org/0000-0001-5195-6254)
- Molly M. Stevens (ORCID: https://orcid.org/0000-0002-7335-266X)
- Vernon LaLone (ORCID: https://orcid.org/0000-0001-6043-8993)
- Krunal Polra
- Ceren Kütahya (ORCID: https://orcid.org/0000-0002-8680-6536)
- Jonathan P. Wojciechowski (ORCID: https://orcid.org/0000-0002-6272-515X)
- Robin J. Shattock (ORCID: https://orcid.org/0000-0002-4936-1718)
- Thomas F. F. Fernandez Debets (ORCID: https://orcid.org/0009-0007-6951-6514)
Institutions
- University of Oxford (GB)
- University of Southampton (GB)
- Imperial College London (GB)
Publication Details
- Journal
- Advanced Materials
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1002/adma.202417290
- Citations
- 2
- Primary Topic
- Nanofabrication and Lithography Techniques
- Type
- article
- Field-Weighted Citation Impact
- 1.35
Funders
- Bill and Melinda Gates Foundation
- Royal Academy of Engineering
- Medical Research Council
- HORIZON EUROPE Marie Sklodowska-Curie Actions