SANS investigation of fungal loosenins reveals substrate-dependent impacts of protein action on the inter-microfibril arrangement of cellulosic substrates
The SANS analysis of PcaLOOL action on holocellulose samples confirms their ability to disrupt cellulose fiber networks and suggests a progression from reducing regular order in the microfibril arrangement to increasing inter-microfibril spacing. The most impactful PcaLOOL, PcaLOOL12, was previously observed to be the most highly expressed loosenin in P. carnosa. Its structural characterization herein reveals its stabilization through two disulfide linkages, and an extended N-terminal region distal to a negatively charged and surface accessible polysaccharide binding groove.
Authors
- Manjula Senanayake (ORCID: https://orcid.org/0000-0002-3139-0625)
- Francisco Vilaplana (ORCID: https://orcid.org/0000-0003-3572-7798)
- Garry W. Buchko (ORCID: https://orcid.org/0000-0002-3639-1061)
- Emma R. Master (ORCID: https://orcid.org/0000-0002-6837-9817)
- Wellington Leite (ORCID: https://orcid.org/0000-0002-5674-3968)
- Hugh O’Neill (ORCID: https://orcid.org/0000-0003-2966-5527)
- Pramod Sivan (ORCID: https://orcid.org/0000-0001-5297-2221)
- Sai Venkatesh Pingali (ORCID: https://orcid.org/0000-0001-7961-4176)
- Zsuzsanna Péter-Szabó
- James Byrnes (ORCID: https://orcid.org/0000-0002-4906-0640)
- Deepika Dahiya (ORCID: https://orcid.org/0000-0002-0085-1951)
Institutions
- Oak Ridge National Laboratory (US)
- Pacific Northwest National Laboratory (US)
- University of Toronto (CA)
- Brookhaven National Laboratory (US)
- Wallenberg Wood Science Center (SE)
- Washington State University (US)
- KTH Royal Institute of Technology (SE)
- Aalto University (FI)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1186/s13068-025-02618-5
- Citations
- 7
- Primary Topic
- Advanced Cellulose Research Studies
- Type
- article
- Field-Weighted Citation Impact
- 4.18
Funders
- National Institutes of Health
- Office of Science
- Horizon 2020 Framework Programme
- National Institute of General Medical Sciences
- Biological and Environmental Research