Periodically Spallated Ag Film as a High-Performing SERS Substrate for Biogenic Amines Detection

This study was focused on development of a rapid and sensitive method for detection of food spoilage marker histamine based on surface-enhanced raman spectroscopy (SERS) using quickly produced laser-textured Ag film as a SERS substrate. Optimization of fs-laser fabrication parameters has reduced the substrate fabrication time to less than a minute. Label-free SERS-detection of histamine adsorbed onto a spallated Ag surface over the concentration range of 10–6 to 10–10 M was demonstrated. The method addresses the need for large number of disposable SERS-substrates required in various biosensing applications.