Bridging the 3D geometrical organisation of white matter pathways across anatomical length scales and species
We used diffusion MRI and x-ray synchrotron imaging on monkey and mice brains to examine the organisation of fibre pathways in white matter across anatomical scales. We compared the structure in the corpus callosum and crossing fibre regions and investigated the differences in cuprizone-induced demyelination in mouse brains versus healthy controls. Our findings revealed common principles of fibre organisation that apply despite the varying patterns observed across species; small axonal fasciculi and major bundles formed laminar structures with varying angles, according to the characteristics of major pathways. Fasciculi exhibited non-straight paths around obstacles like blood vessels, comparable across the samples of varying fibre complexity and demyelination. Quantifications of fibre orientation distributions were consistent across anatomical length scales and modalities, whereas tissue anisotropy had a more complex relationship, both dependent on the field-of-view. Our study emphasises the need to balance field-of-view and voxel size when characterising white matter features across length scales.
Authors
- Mariam Andersson (ORCID: https://orcid.org/0000-0001-7181-3007)
- Tim Salditt (ORCID: https://orcid.org/0000-0003-4636-0813)
- Vedrana Andersen Dahl (ORCID: https://orcid.org/0000-0001-6734-5570)
- Maurice Ptito (ORCID: https://orcid.org/0000-0002-5173-5862)
- Anders Bjorholm Dahl (ORCID: https://orcid.org/0000-0002-0068-8170)
- Maria L. Elkjaer (ORCID: https://orcid.org/0000-0002-8596-142X)
- Alessandro Daducci (ORCID: https://orcid.org/0000-0002-4677-6678)
- Zsolt Illés (ORCID: https://orcid.org/0000-0001-9655-0450)
- Yi He (ORCID: https://orcid.org/0000-0003-0040-9255)
- Mareike Töpperwien
- Alexandra Pacureanu (ORCID: https://orcid.org/0000-0003-2306-7040)
- Hans Martin Kjer (ORCID: https://orcid.org/0000-0001-7900-5733)
- Marina Eckermann (ORCID: https://orcid.org/0000-0001-6418-4310)
- Marco Pizzolato (ORCID: https://orcid.org/0000-0003-2455-4596)
- Tim B. Dyrby (ORCID: https://orcid.org/0000-0003-3361-9734)
- Anna-Lena Robisch
Institutions
- University of Verona (IT)
- Sun Yat-sen University (CN)
- University of Southern Denmark (DK)
- Odense University Hospital (DK)
- European Synchrotron Radiation Facility (FR)
- Copenhagen University Hospital (DK)
- Fifth Affiliated Hospital of Sun Yat-sen University (CN)
- Université de Montréal (CA)
- University of Göttingen (DE)
- Technical University of Denmark (DK)
Publication Details
- Journal
- eLife
- Published
- 2025-02-28
- DOI
- https://doi.org/10.7554/elife.94917.3
- Primary Topic
- Advanced Neuroimaging Techniques and Applications
- Type
- article
- Field-Weighted Citation Impact
- 0.00
Funders
- Scleroseforeningen