Assessment of Analgesic, Antioxidant, and Anti-inflammatory potential of Citrus maxima (Burm.) Merr. Seed solvent fractions on Swiss albino Animal Model

Citrus maxima has been utilized in folk medicine for its various pharmacological characteristics. In-vivoandin-vitroresearchhave been conducted on the methanolic extract of seed. Thus, there is a possibility that some bioactive substances maybe present in the methanolic solvent fractions. N-hexane and dichloromethane were used to fractionate the crude methanolic extract. The objective was to analyze the analgesic, anti-inflammatory, and antioxidant potentials of the dichloromethane (DMS) & n-hexane (NHS) fractions. The writhing test by inducing acetic acidand paw-licking methodbyinducingformalinwere utilized to evaluate the analgesic potential. The 2, 2-diphenyl-1-picryl hydrazyl (DPPH) and hydrogen peroxide scavenging assay were used to measure the antioxidant effect. The paw edema test inducing formalinand carrageenan utilized for assessing the anti-inflammatory efficacy. NHS showed a significance in decreasing the writhing andconsidered to have significant analgesic effect. Compared to the higher (400 mg/kg) dose (**P<0.01), the lower (200 mg/kg) dose had a smaller effect (***P<0.001). At lower doses, DMS demonstrated a minor effect (**P<0.01) and at higher doses, a moderate effect on writhing reduction. NHS demonstrated a lower concentration-dependent scavenging potential of free radicals. At higher concentrations, DMS had a strong antioxidant activity with IC50valuesignificantly lower than that of NHS. In a dose-dependent way, NHS exhibited a considerable high potential (**P<0.01) in reducing paw edema. The current study may provideevidence that the methanolic fractions of seeds could serve as a viable replacement for naturally occurring analgesic, antioxidant, and anti-inflammatory substances.