Spectrometric Analysis of Decrease in Sodium Dodecyl Sulfate (SDS) Concentration by Klebsiella Species Using the Stains-All Dye

Sodium dodecyl sulfate (SDS)-degrading bacteria were isolated from soil and water samples from multiple locations using basal media with 0.05% SDS as the only carbon source. The bacteria were identified using 16S rRNA sequencing and deposited in the NCBI database with specific accession numbers and strain name. There were six bacteria iidentified as Klebsiella variicola MSK86, Klebsiella sp. MSK86, K. quasipneumoniae MSK86, and three K. pneumoniae strains, MSK86, MSK25, and NET12. The potential for decrease in SDS concentration by the bacteria was assessed using “Stains-all.” The SDS standard graph was plotted with different SDS concentration of 0 to 0.06%. The SDS-Stains-all complex exhibited a peak with maximum absorbance at 440 nm. The absorbance of the bacteria-inoculated samples was monitored at 440 nm on every 24 h until the sixth day of incubation. The decrease in concentration of SDS in the medium on day 6 of incubation by K. pneumoniae MSK86, K. variicola MSK86, and Klebsiella sp. MSK86 was found to be 86, 84, and 84%, respectively. K. pneumoniae NET12 demonstrated 76% of decrease. K. pneumoniae MSK25 and K. quasipneumoniae MSK86 were able to survive in the SDS medium, with decrease of 20 and 40%, respectively. The focus of this investigation was finding bacteria that can breakdown SDS, that may contribute to the decrease in the amount of detergent pollution in water.