CRISPR/Cas12a-mediated gene silencing across diverse functional genes demonstrates single gene-specific spacer efficacy in Mycobacterium smegmatis
Our findings reveal the robustness and versatility of the dCas12a-CRISPRi system in M. smegmatis, providing a valuable tool for functional genomics research. This work showcases the potential of the dCas12a-CRISPRi system in investigating essential genes, enabling a deeper understanding of the biology and potential therapeutic targets in mycobacterium species.
Authors
- Devashish Rath (ORCID: https://orcid.org/0000-0002-8204-8440)
- Megha Sodani
- Chitra Seetharam Misra
- Savita Kulkarni
Institutions
- Bhabha Atomic Research Centre (IN)
- Homi Bhabha National Institute (IN)
Publication Details
- Journal
- Journal of Biological Engineering
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1186/s13036-025-00490-3
- Citations
- 1
- Primary Topic
- CRISPR and Genetic Engineering
- Type
- article
- Field-Weighted Citation Impact
- 0.81