In Silico and in Vitro Evaluation of Antihyperlipidemic and Antioxidant Activity of Vigna Mungo Seed Extract

Vigna mungo is a legume which provide significant amount of nutrients. Vigna mungo is one of the important pulse crop in India. It is rich in protein content and traditionally used for treating various disease conditions. The present investigation is conducted to evaluate the potential of Vigna mungo seeds against hyperlipidemia. These compounds were analysed for its activity by molecular docking. The ethanolic extract of both seeds were subjected to qualitative analysis to determine the presence of phytochemicals followed by TLC and HPTLC analysis for the identification of bio constituents. Total phenolic content(TPC) and Total flavonoid content(TFC) of the seed extracts was determined. In vitro antioxidant activity was performed by DPPH assay method and IC50 value was determined. In vitro antihyperlipidemic activity using lipid peroxidation assay method was performed. Ethanolic extract of Vigna mungo seed extracts show the presence of alkaloids, flavonoids, glycosides, phenolic compounds, proteins and terpenoids. Keywords: Vigna mungo, HPTLC, In-silico, Docking, Lipid peroxidation.