Seed Morphology in Vitis Cultivars Related to Hebén

Resolving the genetic relationships between cultivars is one of the objectives of research in viticulture. To this end, both DNA markers and morphological analysis help to identify synonyms and homonyms and to determine the degree of relatedness between cultivars. Results of genetic analysis using single sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs) point to Hebén as the female progenitor of many of the cultivars currently used in viticulture. Here, seed shape is compared between Hebén and genetically related cultivars. An average silhouette derived from seeds of Hebén was used as a model, and the comparisons were made visually and quantitatively by calculation of J-index values (percent similarity of the seeds and the model). Quantification of seed shape by J-index confirms the data of DNA markers supporting different levels of conservation of maternal seed shape in the varieties. Other seed morphological measurements help to explain the basis of the differences in shape between Hebén, genetically related groups and the external group of unrelated cultivars. Curvature analysis in seeds silhouettes confirms the relationship between Hebén and other cultivars and supports the utility of this technique in the analysis of parental relationships.