High correlation between detection of dengue IgG from dried blood spots and serum using an indirect IgG ELISA assay: A validation study in Fortaleza, Brazil
These results validate using DBS instead of serum for detection of prior dengue infection among similar populations in endemic regions, without sacrificing test sensitivity and specificity. The validity of using DBS for ELISA to detect prior dengue infection could have important implications for field-based research. A limitation to this study was that the potential for misclassification due to cross-reactivity (e.g., with Zika virus, Yellow Fever vaccine) was not assessed.
Authors
- Mabel Carabalí (ORCID: https://orcid.org/0000-0002-9171-0483)
- Monica Zahreddine
- Danielle Malta Lima (ORCID: https://orcid.org/0000-0003-2734-6768)
- Kellyanne Abreu
- Kate Zinszer (ORCID: https://orcid.org/0000-0003-1388-1145)
- Laura Pierce (ORCID: https://orcid.org/0009-0004-2012-3515)
- Beatriz Parra (ORCID: https://orcid.org/0000-0002-5371-7449)
- Danielle Ferreira de Oliveira (ORCID: https://orcid.org/0000-0003-1759-5605)
- Valéry Ridde (ORCID: https://orcid.org/0000-0001-9299-8266)
- Katia Charland (ORCID: https://orcid.org/0000-0002-4879-2735)
Institutions
- Universidade Estadual do Ceará (BR)
- Inserm (FR)
- Université Paris Cité (FR)
- Universidade de Fortaleza (BR)
- Sorbonne Université (FR)
- Université Sorbonne Paris Nord (FR)
- Sorbonne Paris Cité (FR)
- Institut de Recherche pour le Développement (FR)
- McGill University (CA)
- Université de Montréal (CA)
- Universidad del Valle (CO)
Publication Details
- Journal
- PLoS neglected tropical diseases
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1371/journal.pntd.0012880
- Citations
- 1
- Primary Topic
- Mosquito-borne diseases and control
- Type
- article
- Field-Weighted Citation Impact
- 1.88
Funders
- Canadian Institutes of Health Research