Investigating the migration of cells with different degrees of metastasis before and after treatment with chemical drugs targeting cytoskeleton structure, steroid and androgen receptor.

Both healthy and cancerous cells have the ability to migrate from one tissue to another. Anticancer drugs can control cell migration through various mechanisms. Although numerous studies have been conducted on cell migration, very few have investigated the effects of drugs with different targets on cell migration. In the present study, the effect of drugs with different targets on the migration of two cell lines, one non-invasive and the other invasive, was examined. Docetaxel drug, which specifically affects the cell’s cytoskeleton structure, namely microtubules and actin filaments, was used to assess the effects of drugs that directly target the cytoskeletal structure. In contrast, the effects of Abiraterone Acetate drug and Bicalutamide drug, which act on steroid, androgen receptor and nucleus proteins respectively, were also investigated. For this purpose, the cells were cultured and treated, followed by scratching with scratcher to create a channel between the cells, and then imaged at various time points. Docetaxel drug had a more significant effect on the migration of PC3 cells compared to the other two drugs, likely due to its targeting of the cell’s cytoskeletal structure. In LNCAP cells, no significant change in the area of the scratched channel was observed, and after some time, this area even increased. This is due to the nature of LNCAP cells, which are non-invasive and inherently have low migration and after a time, these cells are killed by the drug, leading to an increase in the area.