Dragonfly Molecular Diagnostic Platform: A Rapid Point-of-Care Solution for Monkeypox and Skin Lesion Pathogens
be tested for its bactericidal potential using agar diffusion and broth microdilution assays.Discussion: Scorpion venom peptides have been assessed for their potential to act against a broad range of bacteria including Methicillin resistant Staphylococcus aureus (MRSA), Staphylococcus epidermidis, Bacillus subtilis, Micrococcus luteus, Pseudomonas aeruginosa and E. coli.N. tabacum plant system proves to be a better host system for transient expression of recombinant functional antimicrobial peptides such as protegrin-1.Further optimization, extraction and purification of Meucin18 from N. tabacum will be useful in the development of a broad-spectrum antibacterial laboratory reagent. Conclusion:The purified recombinant AMP Meucin18 from N. tabacum will be an efficient antibacterial agent that could be used against a broad range of bacteria.
Authors
- P.H. Holmes
- Diarmuid Stokes (ORCID: https://orcid.org/0000-0003-4319-8048)
- Dr Rebecca P Sumner
- David Ulaeto (ORCID: https://orcid.org/0000-0002-7493-3159)
- Diana D. Cardenas (ORCID: https://orcid.org/0000-0002-6651-6443)
- M. Mirza
- M.Margaret Comer
- David W. Pond
- Dr Luca Miglietta
- Dr Frankie Bolt
- Prof Shiranee Sriskandan
- Dr Carlos Maluquer de Motes
- Dr Jesus Rodriguez-Manzano
- Dr Matthew Cavuto
- Dr Ivana Pennisi
- Dr Sian Lant
Publication Details
- Journal
- International Journal of Infectious Diseases
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1016/j.ijid.2024.107408
- Primary Topic
- Poxvirus research and outbreaks
- Type
- article
- Field-Weighted Citation Impact
- 0.00