An optimized chikungunya virus trans-amplifying RNA vaccine candidate induces potent immune responses with only 1 ng of antigen encoding RNA

to the presence of detoxifying enzyme in the An.gambiae population used in the study. Conclusion:Strategies to manage insecticide resistance particularly against pyrethroids must be scaled up.These include the continued distribution of PBO nets on a wide scale to reach most parts of Chikwawa district and Malawi as a whole.Use of duo nets such as Interceptor G2 and Royal guard nets is also encouraged to manage the growing Insecticide resistance.This will help deter malaria control challenges and overall improve progress towards malaria elimination.