A rapid and efficient reverse genetics method for generating RNA viruses

ally transmitted infections.Malaria prevalence was low in Mbeya CC, Mbeya DC, and Ifakara TC (1%, 1%, and 3% of febrile children, respectively), while much higher in Ulanga and Mlimba (45% and 14%, respectively), with some seasonality (42% from April to November, and 53% from December to March in Ulanga).Pneumonia was more prevalent in Mbeya DC, accounting for 22% of all cases (compared with 8% in Mbeya CC and 11% in the 3 Morogoro districts), with no clear seasonal pattern.Acute watery diarrhea was also more frequent in Mbeya CC and DC, occurring in 19% of the cases, against 9% in the other 3 districts.Microbiological surveillance is ongoing until September 2024; results will be presented during the congress.Discussion: Utilization of a CDSA in health facilities enabled reliable real-time syndromic surveillance.Conclusion: Adding static and mobile targeted microbiological surveillance, triggered by alerts based on clinical and spatiotemporal abnormalities, could potentially allow Tanzania to advance to a smart resource-sparing surveillance system with early detection of outbreaks.