Evaluation of the performance of a multiplex PCR assay for the etiological diagnosis of central nervous system infections at a diagnostic laboratory in South Africa

pg/ml; R2: 0.99), with recovery and reproducibility within the acceptable range, and a low limit of quantification of 1 pg/ml.Discussion: Both the Mabtech Flex and Pro bovine IFN- ELISA assays are deemed suitable for measuring bison IFN- , offering flexibility depending on available resources.These findings indicate the potential of IFN- assays as a diagnostic tool for detecting M. bovis infection in bison populations, contributing to effort s in disease management and conservation. Conclusion:The evaluation of commercial IFN- ELISA assays provides valuable insights further validation is warranted to calculate the assay's cut-off value to confirm their ability to differentiate between M. bovis-infected and uninfected bison to support their role in effective disease surveillance and control measures in bison populations.