Influence of Surfactant Trilaurin Concentration on Synthesis of Hydroxyapatite by Ultrasound Assisted Biomimetic Method

In present study, three different concentrations of a novel surfactant trilaurin has been reported using ultrasound method for synthesizing hydroxyapatite (HAP) powders in presence of the simulated body fluid (SBF) as a solvent in order to investigate the apoptotic cell response. The materials were characterized with FTIR, XRD, SEM, TEM, TGA, BET techniques as well as cytotoxicity (MTT assay) using appropriate cell line. The nanoHAP was characterized for their size and morphology by HRSEM and HRTEM techniques, respectively and were found to have a nanorod like morphology with an average length of 50 nm. The results also validates the cytotoxicity which shows good biocompatibility of nanoHAP powders. The synthesized HAP powders was found to possess improved and uniform size distribution due to the use of non-ionic natural organic modifier (trilaurin). The concentration of trilaurin influences the morphology of the synthesized HAP powders.