A highly accurate nanopore-based sequencing workflow for culture and PCR-free microbial metagenomic profiling of urogenital samples
We set out to develop an unbiased, amplification and culture-free, fully quantitative metagenomic microbiome profiling tool. Our initial observations suggest our method represents a viable alternative to existing molecular research tools employed in the analysis of complex microbiomes.
Authors
- Ben Ferneyhough
- Nick Parkinson (ORCID: https://orcid.org/0000-0001-6336-5654)
- Sam Millington
- Matthew Roddis
- Sean West (ORCID: https://orcid.org/0000-0002-1394-1999)
- Rosalind Schilizzi
- Michael D. Fischer (ORCID: https://orcid.org/0009-0003-6622-236X)
- Chrysolin Clements
- Jess Quirk
Institutions
- National Nuclear Laboratory (GB)
Publication Details
- Journal
- BMC Urology
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1186/s12894-025-01723-9
- Citations
- 3
- Primary Topic
- Gut microbiota and health
- Type
- article
- Field-Weighted Citation Impact
- 2.42