Design and characterization of allantoin-inducible expression systems in budding yeast
In summary, we characterized several allantoin-inducible DAL promoters from budding yeast and further developed a layered allantoin-GAL system that utilizes the DAL2 promoter to regulate the galactose regulon in budding yeast. The resulting allantoin-GAL system could give an impressive ON/OFF ratio that surpassed the traditional P<sub>DAL2</sub>-controlled gene expression. It is anticipated that utilizing our allantoin-inducible system in budding yeast with allantoin as the alternative nitrogen source might favor the low-cost production of biochemicals and pharmaceuticals.
Authors
- Xiaomei Cai (ORCID: https://orcid.org/0000-0003-3782-8875)
- Jifeng Yuan (ORCID: https://orcid.org/0000-0003-1874-190X)
- Jiaxue Ma
- Xueyi Luo (ORCID: https://orcid.org/0000-0002-4975-0895)
- Junyi Wang (ORCID: https://orcid.org/0000-0003-0464-4543)
- Shuo Wang (ORCID: https://orcid.org/0000-0002-1954-2323)
Institutions
- Shenzhen University (CN)
- Xiamen University (CN)
Publication Details
- Journal
- Biotechnology for Biofuels and Bioproducts
- Published
- 2025-02-28
- DOI
- https://doi.org/10.1186/s13068-025-02630-9
- Citations
- 3
- Primary Topic
- Transgenic Plants and Applications
- Type
- article
- Field-Weighted Citation Impact
- 3.70
Funders
- National Natural Science Foundation of China
- Xiamen University
- National Key Research and Development Program of China