A two-column process for bispecific antibody purification based on MabSelect VL resin’s strong byproduct removal capability
The MabSelect VL-based two-column process can be successfully applied to bsAb purification. In addition, it should also be feasible with regular monoclonal antibodies, whose purification is generally less challenging than that of bsAbs. By reducing the downstream process from three columns to two columns, significant savings in terms of time, labor, and materials can be achieved.
Authors
- Yan Wan (ORCID: https://orcid.org/0000-0002-8049-7808)
- Di Wu (ORCID: https://orcid.org/0000-0002-4233-4039)
- Wanyuan Dong
- Penglong Zhang
- Yifeng Li (ORCID: https://orcid.org/0009-0002-6994-9276)
Institutions
- WuXi AppTec (China) (CN)
Publication Details
- Journal
- Journal of Biological Methods
- Published
- 2024-12-12
- DOI
- https://doi.org/10.14440/jbm.2025.0106
- Citations
- 3
- Primary Topic
- Monoclonal and Polyclonal Antibodies Research
- Type
- article
- Field-Weighted Citation Impact
- 1.21